Fetal bovine serum is the highest quality, because the fetus has not been exposed to the outside world, and the antibodies and complement contained in the serum are the least harmful to the cells. This article Weizheng Xiangyu / Dongyi Bio for your analysis of the precipitation of special fetal bovine serum.
(1) Flocs: There are many reasons for this, but the common cause is the deformation of serum lipoproteins and the presence of fibrin in the serum after thawing. These flocculation deposits do not affect the quality of the serum itself. Unless necessary, if you want to reduce these sediments, it is recommended to remove them by centrifugation at 3000 rpm for 5 min, or after centrifugation, add the supernatant to the medium and sterilize and filter.
(2) Observation of "small black spots" under a microscope, and the formation of precipitates is usually increased significantly by the heat-treated serum. Some sediments are observed under the microscope as "small black spots", which are often mistaken for contamination of the serum, and the serum is placed at 37 ° C to cultivate the "microorganisms", but at 37 ° C, the precipitate will be made again. The increase is more likely to be mistaken for the proliferation of microorganisms, but it is detected by the medium in which the bacteria are cultured, and there is no pollution. In general, this small black spot does not affect the growth of cells.
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